Long-term development of reservoir ecosystems - changes in pelagic food webs and their microbial component

Reservoirs differ from lakes mostly in three aspects: (i) water residence times usually are not longer than several months, (ii) horizontal heterogeneity induced by the inflowing river water is more pronounced, and (iii) they are historically much younger than lakes. As a consequence, seasonal and long-term dynamics of pelagic food webs reflect the changes in the catchment and hydrological variations more significantly than in lakes, and an ageing and "maturing" of the reservoir ecosystem might affect the pelagic biomass even several decades after filling. Using long-term data sets on pelagic biomass components from canyonshaped reservoirs of different residence time, the following topics are discussed: (1) changes of pelagic bacteria-phytoplankton - zooplankton abundances during ageing as well as longitudinal changes from the river inflow dowstream to the lacustrine part of a reservoir, (2) long-term changes of pelagic bacteria-phytoplankton-zooplankton abundances and seasonal changes of microbial loop in two reservoirs of different residence times. Periods with relative prevalence of bacterial above zooplankton biomass were detected, mostly coinciding with (or following after) the periods with low phytoplankton to zooplankton ratios.Los embalses difieren de los lagos principalmente en tres aspectos: (i) el tiempo de residencia del agua no suele ser superior a varios meses, (ii) la heterogeneidad horizontal inducida por la entrada de agua fluvial es más marcada, y (iii) son históricamente mucho más jóvenes que los lagos. Como consecuencia de todo ello, las dinámicas estacional y a largo plazo de las redes tróficas pelágicas reflejan los cambios en la cuenca y las variaciones hidrológicas más significativamente que en los lagos, y el envejecimiento y "madurez" del ecosistema del embalse podría afectar a la biomasa pelágica incluso varias décadas después de su llenado. Mediante el análisis de largas series de datos sobre la biomasa de componentes pelágicos en embalses con sección en forma de cañón y diferentes tiempos de residencia, se discuten los siguientes aspectos: (1) cambios en las abundancias de bacterias-fitoplancton-zooplancton pelágicos durante el envejecimiento del embalse, así como cambios longitudinales desde la entrada de los ríos hasta la zona lacustre del embalse, (2) cambios a largo plazo en las abundancias de bacterias-fitoplancton-zooplancton pelágicos y cambios estacionales del bucle microbiano en dos embalses con diferentes tiempos de residencia. Se detectaron períodos con un predominio relativo de la biomasa bacteriana sobre la biomasa de zooplancton, coincidiendo principalmente con (o justamente después de) los períodos con bajas relaciones fitoplancton / zooplancton

Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens

<p>Abstract</p> <p>Background</p> <p><it>Eimeria </it>parasites can cause the disease coccidiosis in poultry and even subclinical infection can incur economic loss. Diagnosis of infection predominantly relies on traditional techniques including lesion scoring and faecal microscopy despite the availability of sensitive molecular assays, largely due to cost and the requirement for specialist equipment. Despite longstanding proven efficacy these traditional techniques demand time and expertise, can be highly subjective and may under-diagnose subclinical disease. Recognition of the tight economic margins prevailing in modern poultry production and the impact of avian coccidiosis on poverty in many parts of the world has highlighted a requirement for a panel of straightforward and sensitive, but cost-effective, <it>Eimeria </it>species-specific diagnostic assays.</p> <p>Results</p> <p>Loop-mediated isothermal amplification (LAMP) is an uncomplicated, quick and relatively inexpensive diagnostic tool. In this study we have developed a panel of species-specific LAMP assays targeting the seven <it>Eimeria </it>species that infect the chicken. Each assay has been shown to be genuinely species-specific with the capacity to detect between one and ten eimerian genomes, equivalent to less than a single mature schizont. Development of a simple protocol for template DNA preparation from tissue collected post mortem with no requirement for specialist laboratory equipment supports the use of these assays in routine diagnosis of eimerian infection. Preliminary field testing supports this hypothesis.</p> <p>Conclusions</p> <p>Development of a panel of sensitive species-specific LAMP assays introduces a valuable new cost-effective tool for use in poultry husbandry.</p

A 10-micron Search for Inner-Truncated Disks Among Pre-Main-Sequence Stars With Photometric Rotation Periods

We use mid-IR (primarily 10 $\mu$m) photometry as a diagnostic for the presence of disks with inner cavities among 32 pre-main sequence stars in Orion and Taurus-Auriga for which rotation periods are known and which do not show evidence for inner disks at near-IR wavelengths. Disks with inner cavities are predicted by magnetic disk-locking models that seek to explain the regulation of angular momentum in T Tauri stars. Only three stars in our sample show evidence for excess mid-IR emission. While these three stars may possess truncated disks consistent with magnetic disk-locking models, the remaining 29 stars in our sample do not. Apparently, stars lacking near-IR excesses in general do not possess truncated disks to which they are magnetically coupled. We discuss the implications of this result for the hypothesis of disk-regulated angular momentum. Evidently, young stars can exist as slow rotators without the aid of present disk-locking, and there exist very young stars already rotating near breakup velocity whose subsequent angular momentum evolution will not be regulated by disks. Moreover, we question whether disks, when present, truncate in the manner required by disk-locking scenarios. Finally, we discuss the need for rotational evolution models to take full account of the large dispersion of rotation rates present at 1 Myr, which may allow the models to explain the rotational evolution of low-mass pre-main sequence stars in a way that does not depend upon braking by disks.Comment: 20 pages, 4 figure

Results of the ROTOR-program. I. The long-term photometric variability of classical T Tauri stars

We present a unique, homogeneous database of photometric measurements for Classical T Tauri stars extending up to 20 years. The database contains more than 21,000 UBVR observations of 72 CTTs. All the data were collected within the framework of the ROTOR-program at Mount Maidanak Observatory (Uzbekistan) and together they constitute the longest homogeneous, accurate record of TTS variability ever assembled. We characterize the long term photometric variations of 49 CTTs with sufficient data to allow a robust statistical analysis and propose an empirical classification scheme. Several patterns of long term photometric variability are identified. The most common pattern, exhibited by a group of 15 stars which includes T Tau itself, consists of low level variability (Delta(V)<=0.4mag) with no significant changes occurring from season to season over many years. A related subgroup of 22 stars exhibits a similar stable long term variability pattern, though with larger amplitudes (up to Delta(V)~1.6 mag). Besides these representative groups, we identify three smaller groups of 3-5 stars each which have distinctive photometric properties. The long term variability of most CTTs is fairly stable and merely reflects shorter term variability due to cold and hot surface spots. Only a small fraction of CTTs undergo significant brightness changes on the long term (months, years), which probably arise from slowly varying circumstellar extinction.Comment: 16 pages, 11 figures. Astron. Astrophys., in pres

Biomic Specialization and Speciation Rates in Ruminants (Cetartiodactyla, Mammalia): A Test of the Resource-Use Hypothesis at the Global Scale

The resource-use hypothesis proposed by E.S. Vrba predicts that specialist species have higher speciation and extinction rates than generalists because they are more susceptible to environmental changes and vicariance. In this work, we test some of the predictions derived from this hypothesis on the 197 extant and recently extinct species of Ruminantia (Cetartiodactyla, Mammalia) using the biomic specialization index (BSI) of each species, which is based on its distribution within different biomes. We ran 10000 Monte Carlo simulations of our data in order to get a null distribution of BSI values against which to contrast the observed data. Additionally, we drew on a supertree of the ruminants and a phylogenetic likelihood-based method (QuaSSE) for testing whether the degree of biomic specialization affects speciation rates in ruminant lineages. Our results are consistent with the predictions of the resource-use hypothesis, which foretells a higher speciation rate of lineages restricted to a single biome (BSI = 1) and higher frequency of specialist species in biomes that underwent high degree of contraction and fragmentation during climatic cycles. Bovids and deer present differential specialization across biomes; cervids show higher specialization in biomes with a marked hydric seasonality (tropical deciduous woodlands and schlerophyllous woodlands), while bovids present higher specialization in a greater variety of biomes. This might be the result of divergent physiological constraints as well as a different biogeographic and evolutionary history

Dynamical Mass Constraints on Low-Mass Pre-Main-Sequence Stellar Evolutionary Tracks: An Eclipsing Binary in Orion with a 1.0 Msun Primary and an 0.7 Msun Secondary

We report the discovery of a double-lined, spectroscopic, eclipsing binary in the Orion star-forming region. We analyze the system spectroscopically and photometrically to empirically determine precise, distance-independent masses, radii, effective temperatures, and luminosities for both components. The measured masses for the primary and secondary, accurate to ~1%, are 1.01 Msun and 0.73 Msun, respectively; thus the primary is a definitive pre-main-sequence solar analog, and the secondary is the lowest-mass star yet discovered among pre-main-sequence eclipsing binary systems. We use these fundamental measurements to test the predictions of pre-main-sequence stellar evolutionary tracks. None of the models we examined correctly predict the masses of the two components simultaneously, and we implicate differences between the theoretical and empirical effective temperature scales for this failing. All of the models predict the observed slope of the mass-radius relationship reasonably well, though the observations tend to favor models with low convection efficiencies. Indeed, considering our newly determined mass measurements together with other dynamical mass measurements of pre-main-sequence stars in the literature, as well as measurements of Li abundances in these stars, we show that the data strongly favor evolutionary models with inefficient convection in the stellar interior, even though such models cannot reproduce the properties of the present-day Sun.Comment: Accepted by Ap

Исследование влияния технологического процесса изготовления обмоток на дефектность корпусной изоляции асинхронных двигателей

В работе проведено исследование влияния колебаний режимов работы статорообмоточных станков WST-660 и пазоизолировочных станков ИПС-3 на дефектность корпусной изоляции. Получены математические модели дефектообразования в корпусной изоляции обмотки с учетом режимов работы технологического оборудования и качества материала корпусной изоляции в состоянии поставки. Установлено, что изменением режимов работы технологического оборудования можно добиться требуемого качества корпусной изоляции при максимальной производительности оборудования